mouse brain vascular pericyte  (iXCells Biotechnologies)

Journal: Journal of Inflammation (London, England)

Article Title: Contribution of brain pericytes to neuroinflammation following repetitive head trauma

doi: 10.1186/s12950-025-00439-9

Figure Lengend Snippet: Timeline of the brain injury paradigm and tissue collection for the mouse studies. C57BL/6 mice at 3 months of age received 2 injuries per week for 3 months. Mice were euthanized and tissue was collected at 24 h, 3 months, 6 months and 12 months after the final brain injury or anesthesia exposure for r-sham control mice. Moreover, for pericyte isolation studies, mice were fed with phenytoin for 4 weeks before euthanasia

Article Snippet: Mouse brain vascular pericytes (MBVP) (Cat No. M1200-57) were purchased from ScienCell Research Laboratories.

Techniques: Control, Isolation

Journal: Journal of Inflammation (London, England)

Article Title: Contribution of brain pericytes to neuroinflammation following repetitive head trauma

doi: 10.1186/s12950-025-00439-9

Figure Lengend Snippet: Inflammatory insult does not have a cytotoxic effect on mouse brain vascular pericytes but drives pericytes towards an inflammatory phenotype in vitro. A PDGFRβ expression levels after treatment with a combination of the three cytokines for 2 and 24 h. Pericyte lysates were analyzed by a PDGFRβ ELISA assay. B Pericytes were treated with each individual cytokine (100 ng/ml) or with a combination of the three cytokines for 2 and 24 h. The MTT assay was used to measure cytotoxicity. C Representative western blot images of pericytes treated with a combination of the three cytokines for 2 and 24 h. Bands were quantified with ImageLab and normalized to actin. D Total MMP9 levels in pericytes treated with a combination of the three cytokines for 2 and 24 h. Pericyte lysates were analyzed by a total MMP9 ELISA assay. All results are presented as mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 Multiple comparisons (ANOVA)

Article Snippet: Mouse brain vascular pericytes (MBVP) (Cat No. M1200-57) were purchased from ScienCell Research Laboratories.

Techniques: In Vitro, Expressing, Enzyme-linked Immunosorbent Assay, MTT Assay, Western Blot

Journal: Journal of Inflammation (London, England)

Article Title: Contribution of brain pericytes to neuroinflammation following repetitive head trauma

doi: 10.1186/s12950-025-00439-9

Figure Lengend Snippet: PDGF-BB and/or cytokine treatment increases pericyte proliferation in vitro. The percentage of Edu+ cells was determined by image analysis using ImageJ Software. All results are presented as mean ± SEM.* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 Multiple comparisons (ANOVA). Scale bar 40 μm

Article Snippet: Mouse brain vascular pericytes (MBVP) (Cat No. M1200-57) were purchased from ScienCell Research Laboratories.

Techniques: In Vitro, Software

Journal: Journal of Inflammation (London, England)

Article Title: Contribution of brain pericytes to neuroinflammation following repetitive head trauma

doi: 10.1186/s12950-025-00439-9

Figure Lengend Snippet: PDGF-BB and cytokines alters PDGFRβ expression and signaling in inflamed pericytes. Pericytes were treated with cytokines for 24 h with and without PDGF-BB. A Pericyte lysates were analyzed using an ELISA assay. B Pericytes were fixed and immunostained for pPDGFRβ and quantification of intracellular pPDGFRβ was performed measuring the CTCF (Corrected Total Cell Fluorescence), which includes both nuclear and cytoplasmic levels. Representative images of pPDGFRβ (magenta) and DAPI (blue) for each treated group are shown. All results are presented as mean ± SEM.* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 Multiple comparisons (ANOVA). Scale bar 40 μm

Article Snippet: Mouse brain vascular pericytes (MBVP) (Cat No. M1200-57) were purchased from ScienCell Research Laboratories.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Fluorescence

Journal: Journal of Inflammation (London, England)

Article Title: Contribution of brain pericytes to neuroinflammation following repetitive head trauma

doi: 10.1186/s12950-025-00439-9

Figure Lengend Snippet: PDGF-BB stimulation can prevent or mitigate the activated immune-response of pericytes upon exposure to pro-inflammatory molecules. Pericytes were treated with cytokines and/or vehicle for 24 h and PDGF-BB stimulation was given prior or following the inflammatory insult. Cells were fixed and immunostained for A VCAM1, B STAT1, C pAkt, and D NFkB. E Cell lysates were analyzed by a total MMP9 ELISA assay. F Representative images for each treatment group with DAPI (blue) are shown. All results are presented as mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 Multiple comparisons (ANOVA). Scale bar 40 μm

Article Snippet: Mouse brain vascular pericytes (MBVP) (Cat No. M1200-57) were purchased from ScienCell Research Laboratories.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Journal of Inflammation (London, England)

Article Title: Contribution of brain pericytes to neuroinflammation following repetitive head trauma

doi: 10.1186/s12950-025-00439-9

Figure Lengend Snippet: Pericyte inflammatory protein secretion following cytokine insult and PDGF-BB stimulation. Conditioned media was collected from cultured mouse brain vascular pericytes (MBVP) treated with both cytokines and/or PDGF-BB for 24 h and probed for immunomodulatory factors using the pro-inflammatory panel 1 MSD assay. All results are presented as mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 Multiple comparisons (ANOVA)

Article Snippet: Mouse brain vascular pericytes (MBVP) (Cat No. M1200-57) were purchased from ScienCell Research Laboratories.

Techniques: Cell Culture

Journal: Journal of Inflammation (London, England)

Article Title: Contribution of brain pericytes to neuroinflammation following repetitive head trauma

doi: 10.1186/s12950-025-00439-9

Figure Lengend Snippet: Figure 9. Inflammator y secretome profiles of brain pericytes following TBI. Primary mouse pericytes were isolated by magnetic cell sorting from r-sham and r-mTBI mice (6 months post-injury) following 4 weeks of treatment with phenytoinadministered in mouse chow. After 3-5 days, conditioned media from the isolated pericytes were collected and probed for immunomodulatory factors using the pro-inflammatory panel 1 MSD assay. All results are presented as mean ± SEM * p < 0.05; ** p < 0.01; Multiple comparisons (ANOVA) and # p < 0.05; ## p < 0.01; t-test

Article Snippet: Mouse brain vascular pericytes (MBVP) (Cat No. M1200-57) were purchased from ScienCell Research Laboratories.

Techniques: Isolation, FACS